β-Actin Rabbit Monoclonal Antibody for Normalization 0.1mL

409,60

The β-actin primary antibody can be used as an internal loading control (ILC) for normalization.

The expression of β-actin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, β-actin primary antibodies can be used for the detection of β-actin when performing two-color detection.

Detect β-Actin Rabbit Primary Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Available on backorder

SKU: 926-42210 Categories: ,

Reactivity and Specificity

β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties β-Actin Rabbit Monoclonal Antibody (P/N 926-42210)
Species Cross-Reactivity Human, mouse, rat, monkey
Target Molecular Weight 45 kDa
Isotype Rabbit IgG
Specificity/Sensitivity May cross-react with γ-actin (cytoplasmic isoform)
Immunogen A synthetic peptide (KLH-coupled) that corresponds to the residues near the amino-terminus of human β-actin
Tested Applications Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)
Weight 0.002 kg
Volume

0.1ml

Preservation

-20 ° C

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