α-Tubulin Mouse Monoclonal Antibody for Normalization 0.1mL

α-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.

The expression of α-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, α-tubulin primary antibodies can be used for the detection of α-tubulin when performing two-color detection.

Detect α-Tubulin Mouse Monoclonal Antibody with IRDye^® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.

α-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties	α-Tubulin Mouse Monoclonal Antibody (P/N 926-42213)
Species Cross-Reactivity	Human, mouse, rat, monkey
Target Molecular Weight	52 kDa
Isotype	Mouse IgG₁
Specificity/Sensitivity	Detects endogenous levels of total α-tubulin protein. α/β-tubulin heterodimers form the tubulin subunit common to all eukaryotic cells.
Immunogen	Full-length chicken α-tubulin (purified from brain extracts)
Tested Applications	Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)