IRDye® 680LT NHS Ester (0.5 mg)

206,40

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence.

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

 

Note: IRDye 680LT dye products should not be used for small animal in vivo imaging or In ‑ Cell Western Assays. The higher level of fluorescent intensity creates a high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.

IRDye® 800CW NHS Ester reaction
NHS Ester reacts with a primary aliphatic amine, such as lysine.
IRDye® 800CW NHS Ester amide bond
Dye label is attached through amide bond.

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Dye Structure

IRDye® 680LT NHS Ester Structure

Properties

  • Chemical Formula: C 54 H 52 N 3 Na 3 O 22 S 6
  • Molecular Weight: 1402.32 g / mol
  • Exact Mass: 1401.09

Absorption and Emission Spectra

Solvent Ext. Coeff. (M -1 cm -1 ) Absorption Maxima Emission Maxima
Methanol 250,000 680 nm 694 nm
1X PBS 250,000 676 nm 693 nm

Absorption and Emission Spectra in 1X PBS

IRDye® 680LT Absorption and Emission Spectra
Kaal 0,002 kg
Volume

0.5 mg

Preservation

-20 ° C

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