IRDye® 680RD Protein Labeling Kits (high molecular weight)

IRDye 680RD Protein Labeling Kits can be used to label antibodies and other proteins for Western blotting, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section applications.

High Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 45 – 200 kDa.

This kit contains:

• • 3 x 0.1 mg IRDye 680RD Reactive Dye vials
  • 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9
  • 25 mL 1X PBS
  • 0.5 mL ultra-pure water
  • 3 x Pierce^® Zeba™ Desalting Spin Columns (P/N 89891)

Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.

• Pierce Zeba Desalting Spin column instructions
• Protocol for IRDye 680RD Protein Labeling Kit – High MW