β-Actin Mouse Monoclonal Antibody for Normalization 0.1mL

The β-actin primary antibody can be used as an internal loading control (ILC) for normalization.

The expression of β-actin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, β-actin primary antibodies can be used for the detection of β-actin when performing two-color detection.

Detect β-Actin Mouse Primary Antibody with IRDye^® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.

β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties	β-Actin Mouse Monoclonal Antibody (P/N 926-42212)
Species Cross-Reactivity	Human, mouse, rat, monkey, hamster
Target Molecular Weight	45 kDa
Isotype	Mouse IgG_2b
Specificity/Sensitivity	Detects endogenous levels of β-actin protein
Immunogen	A synthetic peptide (KLH-coupled) that corresponds to the residues near the amino-terminus of human β-actin
Tested Applications	Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)