IRDye® 680LT Streptavidin 0.1mg


Application Suggested Range Tween 20* SDS*
Odyssey®Western blot detection 1:1,000 – 1:5,000 0.1 – 0.2% (v/v) 0.02 – 0.1% (v/v)
Other User optimized User optimized User optimized

Saadaval järeltellimisel

Tootekood: 925-68031 Kategooriad: ,

IRDye 680LT Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.

To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.

For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.

Kaal 0,002 kg

0.1 mg



Shopping Cart