β-Tubulin Rabbit Polyclonal Antibody for Normalization 0.1mL

The β-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.

The expression of β-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, β-tubulin primary antibodies can be used for the detection of β-tubulin when performing two-color detection.

Detect β-Tubulin Rabbit Polyclonal Antibody with IRDye^® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

β-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties	β-Tubulin Rabbit Polyclonal Antibody (P/N 926-42211)
Species Cross-Reactivity	Human, mouse, rat, monkey, bovine
Target Molecular Weight	55 kDa
Isotype	Rabbit IgG
Specificity/Sensitivity	Detects endogenous levels of total β-tubulin protein. Does not cross-react with recombinant α-tubulin.
Immunogen	A synthetic peptide (KLH-coupled) that corresponds to the sequence of total β-tubulin, and does not cross-react with recombinant α-tubulin
Tested Applications	Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)