Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG1 covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG1. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG2a, IgG2b, IgG3, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.
