IRDye® 800RS NHS Ester (0.5 mg)


IRDye 800RS infrared dye is an excellent choice for nucleic acid applications. This near-infrared fluorescence dye has good water solubility, but low salt tolerance. It is more hydrophobic than IRDye 800CW.

IRDye 800RS NHS ester can be used to label the primary and secondary amino groups of DNA and RNA. Nucleic acids that have been labeled with IRDye 800RS can be easily purified by reverse-phase chromatography.

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Dye Structure

IRDye 800RS NHS Ester structure


  • Chemical Formula: C50H56N3NaO11S2
  • Molecular Weight: 962.12 g/mol
  • Exact Mass: 961.33

Absorption and Emission Spectra

Solvent Ext. Coeff. (M-1cm-1) Absorption Maxima Emission Maxima Stokes Shift
Methanol 300,000 770 nm 786 nm 16 nm
Water 200,000 767 nm 786 nm 19 nm
1X PBS 200,000 767 nm 786 nm 19 nm
PBS: Methanol 270,000 770 nm 786 nm 16 nm

Absorption and Emission Spectra in 1X PBS

IRDye 800RS Absorption and Emission Spectra

0.5 mg




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