Mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.
The Odyssey EMSA Kit, coupled with IRDye 700 EMSA oligonucleotides, is an excellent alternative method to radioisotopic and chemiluminescent detection methods for EMSA analysis and visualization1, 2. Using IRDye EMSA reagents, assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even need to be removed from the glass plates for imaging.